Journal: Communications biology

Article Title: Single-cell transcriptomes reveal a molecular link between diabetic kidney and retinal lesions.

doi: 10.1038/s42003-023-05300-4

Figure Lengend Snippet: Fig. 4 Transcriptomes of HMCs and HRPCs in response to control or AGEs treatment. a The overlap of highly expressed genes in the normal control groups of HMCs and HRPCs. b Shared enriched GO pathways between HMCs and HRPCs, sorted by p-value. c Volcano plot shows DEGs in HMCs and HRPCs treated with AGEs. The red dots indicate upregulated genes while blue dots indicate downregulated genes. d Expression levels of CXCL1, CXCL2, CXCL3, CXCL5, CXCL6 and CXCL8 were measured by real-time PCR and ELISA between control and AGEs groups. e Significantly enriched KEGG pathways of DEGs in both HMCs and HRPCs treated with AGEs, the size of circle represents genes number enriched in pathways, the darker color of circle indicates a smaller p-value. f The chemokine scores of MCs and RPCs in wt and db/db mice. g The proportions of infiltrating neutrophils and macrophages in the glomeruli of wt and db/db mice. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: Chemokines in the cell culture supernatant were assayed using enzyme-linked immunosorbent assay (ELISA) kits (EK0722, EK0728, EK0359 and EK0413 from BOSTER, Wuhan, China and EK1264 and EK1265 from MULTI SCIENCES, Hangzhou, China) following the manufacturer’s instructions.

Techniques: Control, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 2. RT-PCR analysis of HLA-D gene transcription in the four melanoma cell lines and in the BUA cell line. The number of amplification cycles is given for each primer set in Table I. cDNAs either from cells treated for 48 h with 250 U/ml IFN-g (1) or from untreated cells (2) and the PCR products were analyzed on ethidium bromide-stained agarose gels. To assess that equal amounts of cDNA were used, a 16-cycle amplification was performed on the cDNAs with GAPDH primers, and the correspond- ing Dig-labeled PCR products were stained with CSPD.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Reverse Transcription Polymerase Chain Reaction, Staining, Labeling

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 1. Cell surface expression of the HLA-DR molecules in the 42/95 and HT144 melanoma cell lines examined by indirect immunoflu- orescence. The cells were either treated with 250 U/ml IFN-g for 48 h or untreated as indicated on the figure (light histograms). The dark histogram corresponds to the isotypic control. The L243 mAb was used to detect the expression of HLA-DR molecules by cytofluorometry.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Expressing, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 4. RT-PCR analysis of CIITA transcript expression in the dif- ferent melanoma cell lines and in the BUA cell line through a 33-cycle amplification procedure. cDNAs were prepared from cells either untreated (2) or treated (1) with 250 U/ml IFN-g for 6 h. The amplification products were detected on ethidium bromide-stained gels. To assess that equal amounts of cDNAs were used, a 16-cycle amplification was performed on the cDNAs with GAPDH-specific primers, and the Dig-labeled amplifica- tion products were stained with CSPD.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Staining, Labeling

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 5. RT-PCR analysis of IRF-1 transcript expression in the dif- ferent melanoma cell lines and in the BUA cell line. cDNA quantification with GAPDH-specific primers was performed on Dig-labeled amplified products.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Labeling